Development of Dot – Enzyme Linked Immunosorbent Assay for Detection of Antibodies to Infectious Bursal Disease, Hydropericardium Syndrome and Chicken Anaemia Viruses

A rapid and sensitive dot-ELISA test was developed for the simultaneous detection of antibodies against infectious bursal disease (IBD), hydropericardium syndrome (HPS) and chicken anaemia (CAV) viruses in a single serum sample. The developed dot-ELISA was compared with the virus neutralization test (VNT). For IBD and HPS, viruses isolated and purified from field outbreak materials were used while VP1 ORF3 recombinant protein expressed in DH5α E.coli was used for CAV as coating antigens. Sera samples from 302 breeder chicken were screened for antibodies to the three viruses. A total of 195, 205 and 104 serum samples were found positive for IBDV, HPSV and CAV respectively. By comparing with VNT sensitivity, specificity and accuracy of the test were calculated. The dot-ELISA test was found to be highly sensitive, specific with a K value of 0.99 and hence could be very useful for serological diagnosis of IBDV, HPS and CAV in a single serum sample.